Cell type analysis of functional fetal dopamine cell suspension transplants in the striatum and substantia nigra of patients with Parkinson's disease. Commentary
Identifieur interne : 002B43 ( Main/Exploration ); précédent : 002B42; suivant : 002B44Cell type analysis of functional fetal dopamine cell suspension transplants in the striatum and substantia nigra of patients with Parkinson's disease. Commentary
Auteurs : Deniz Kirik [Suède] ; Anders Björklund [Suède] ; Ivar Mendez [Canada] ; Rosario Sanchez-Pernaute [États-Unis] ; Oliver Cooper [États-Unis] ; Angel Vinuela [États-Unis] ; Daniela Ferrari [États-Unis] ; Lars Björklund [États-Unis] ; Alain Dagher [Canada] ; Ole Isacson [États-Unis]Source :
- Brain [ 0006-8950 ] ; 2005.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
Abstract
We report the first post-mortem analysis of two patients with Parkinson's disease who received fetal midbrain transplants as a cell suspension in the striatum, and in one case also in the substantia nigra. These patients had a favourable clinical evolution and positive 18F-fluorodopa PET scans and did not develop motor complications. The surviving transplanted dopamine neurons were positively identified with phenotypic markers of normal control human substantia nigra (n = 3), such as tyrosine hydroxylase, G-protein-coupled inward rectifying current potassium channel type 2 (Girk2) and calbindin. The grafts restored the cell type that provides specific dopaminergic innervation to the most affected striatal regions in the parkinsonian brain. Such transplants were able to densely reinnervate the host putamen with new dopamine fibres. The patients received only 6 months of standard immune suppression, yet by post-mortem analysis 3-4 years after surgery the transplants appeared only mildly immunogenic to the host brain, by analysis of microglial CD45 and CD68 markers. This study demonstrates that, using these methods, dopamine neuronal replacement cell therapy can be beneficial for patients with advanced disease, and that changing technical approaches could have a favourable impact on efficacy and adverse events following neural transplantation.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000900
- to stream PascalFrancis, to step Curation: 000423
- to stream PascalFrancis, to step Checkpoint: 000815
- to stream Main, to step Merge: 002E83
- to stream Main, to step Curation: 002B43
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Cell type analysis of functional fetal dopamine cell suspension transplants in the striatum and substantia nigra of patients with Parkinson's disease. Commentary</title><author><name sortKey="Kirik, Deniz" sort="Kirik, Deniz" uniqKey="Kirik D" first="Deniz" last="Kirik">Deniz Kirik</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Wallenberg Neuroscience Center Department of Experimental Medical Science Lund University, BMC A11</s1><s2>22466, Lund</s2><s3>SWE</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>22466, Lund</wicri:noRegion></affiliation></author><author><name sortKey="Bjorklund, Anders" sort="Bjorklund, Anders" uniqKey="Bjorklund A" first="Anders" last="Björklund">Anders Björklund</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Wallenberg Neuroscience Center Department of Experimental Medical Science Lund University, BMC A11</s1><s2>22466, Lund</s2><s3>SWE</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>22466, Lund</wicri:noRegion></affiliation></author><author><name sortKey="Mendez, Ivar" sort="Mendez, Ivar" uniqKey="Mendez I" first="Ivar" last="Mendez">Ivar Mendez</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Dalhousie University and Queen Elizabeth II Health Science Center, Division of Neurosurgery and Neuroscience</s1><s2>Halifax</s2><s3>CAN</s3><sZ>3 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Halifax</wicri:noRegion></affiliation></author><author><name sortKey="Sanchez Pernaute, Rosario" sort="Sanchez Pernaute, Rosario" uniqKey="Sanchez Pernaute R" first="Rosario" last="Sanchez-Pernaute">Rosario Sanchez-Pernaute</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Cooper, Oliver" sort="Cooper, Oliver" uniqKey="Cooper O" first="Oliver" last="Cooper">Oliver Cooper</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Vinuela, Angel" sort="Vinuela, Angel" uniqKey="Vinuela A" first="Angel" last="Vinuela">Angel Vinuela</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Ferrari, Daniela" sort="Ferrari, Daniela" uniqKey="Ferrari D" first="Daniela" last="Ferrari">Daniela Ferrari</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Bjorklund, Lars" sort="Bjorklund, Lars" uniqKey="Bjorklund L" first="Lars" last="Björklund">Lars Björklund</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Dagher, Alain" sort="Dagher, Alain" uniqKey="Dagher A" first="Alain" last="Dagher">Alain Dagher</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>McGill University and Montreal Neurological Institute, McConnel Brain Imaging Centre</s1><s2>Montreal</s2><s3>CAN</s3><sZ>9 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Isacson, Ole" sort="Isacson, Ole" uniqKey="Isacson O" first="Ole" last="Isacson">Ole Isacson</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">05-0329433</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 05-0329433 INIST</idno><idno type="RBID">Pascal:05-0329433</idno><idno type="wicri:Area/PascalFrancis/Corpus">000900</idno><idno type="wicri:Area/PascalFrancis/Curation">000423</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000815</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000815</idno><idno type="wicri:doubleKey">0006-8950:2005:Kirik D:cell:type:analysis</idno><idno type="wicri:Area/Main/Merge">002E83</idno><idno type="wicri:Area/Main/Curation">002B43</idno><idno type="wicri:Area/Main/Exploration">002B43</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Cell type analysis of functional fetal dopamine cell suspension transplants in the striatum and substantia nigra of patients with Parkinson's disease. Commentary</title><author><name sortKey="Kirik, Deniz" sort="Kirik, Deniz" uniqKey="Kirik D" first="Deniz" last="Kirik">Deniz Kirik</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Wallenberg Neuroscience Center Department of Experimental Medical Science Lund University, BMC A11</s1><s2>22466, Lund</s2><s3>SWE</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>22466, Lund</wicri:noRegion></affiliation></author><author><name sortKey="Bjorklund, Anders" sort="Bjorklund, Anders" uniqKey="Bjorklund A" first="Anders" last="Björklund">Anders Björklund</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Wallenberg Neuroscience Center Department of Experimental Medical Science Lund University, BMC A11</s1><s2>22466, Lund</s2><s3>SWE</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Suède</country><wicri:noRegion>22466, Lund</wicri:noRegion></affiliation></author><author><name sortKey="Mendez, Ivar" sort="Mendez, Ivar" uniqKey="Mendez I" first="Ivar" last="Mendez">Ivar Mendez</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Dalhousie University and Queen Elizabeth II Health Science Center, Division of Neurosurgery and Neuroscience</s1><s2>Halifax</s2><s3>CAN</s3><sZ>3 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Halifax</wicri:noRegion></affiliation></author><author><name sortKey="Sanchez Pernaute, Rosario" sort="Sanchez Pernaute, Rosario" uniqKey="Sanchez Pernaute R" first="Rosario" last="Sanchez-Pernaute">Rosario Sanchez-Pernaute</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Cooper, Oliver" sort="Cooper, Oliver" uniqKey="Cooper O" first="Oliver" last="Cooper">Oliver Cooper</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Vinuela, Angel" sort="Vinuela, Angel" uniqKey="Vinuela A" first="Angel" last="Vinuela">Angel Vinuela</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Ferrari, Daniela" sort="Ferrari, Daniela" uniqKey="Ferrari D" first="Daniela" last="Ferrari">Daniela Ferrari</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Bjorklund, Lars" sort="Bjorklund, Lars" uniqKey="Bjorklund L" first="Lars" last="Björklund">Lars Björklund</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author><author><name sortKey="Dagher, Alain" sort="Dagher, Alain" uniqKey="Dagher A" first="Alain" last="Dagher">Alain Dagher</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>McGill University and Montreal Neurological Institute, McConnel Brain Imaging Centre</s1><s2>Montreal</s2><s3>CAN</s3><sZ>9 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Isacson, Ole" sort="Isacson, Ole" uniqKey="Isacson O" first="Ole" last="Isacson">Ole Isacson</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Harvard University and McLean Hospital, NINDS Udall Parkinson's Disease Research Center of Excellence</s1><s2>Belmont, MA</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Belmont, MA</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Brain</title><title level="j" type="abbreviated">Brain</title><idno type="ISSN">0006-8950</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Brain</title><title level="j" type="abbreviated">Brain</title><idno type="ISSN">0006-8950</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Dopamine</term><term>Functional analysis</term><term>Human</term><term>Locus niger</term><term>Nervous system diseases</term><term>Neuron</term><term>Parkinson disease</term><term>Transplantation</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Système nerveux pathologie</term><term>Parkinson maladie</term><term>Analyse fonctionnelle</term><term>Dopamine</term><term>Locus niger</term><term>Homme</term><term>Transplantation</term><term>Neurone</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report the first post-mortem analysis of two patients with Parkinson's disease who received fetal midbrain transplants as a cell suspension in the striatum, and in one case also in the substantia nigra. These patients had a favourable clinical evolution and positive <sup>18</sup>F-fluorodopa PET scans and did not develop motor complications. The surviving transplanted dopamine neurons were positively identified with phenotypic markers of normal control human substantia nigra (n = 3), such as tyrosine hydroxylase, G-protein-coupled inward rectifying current potassium channel type 2 (Girk2) and calbindin. The grafts restored the cell type that provides specific dopaminergic innervation to the most affected striatal regions in the parkinsonian brain. Such transplants were able to densely reinnervate the host putamen with new dopamine fibres. The patients received only 6 months of standard immune suppression, yet by post-mortem analysis 3-4 years after surgery the transplants appeared only mildly immunogenic to the host brain, by analysis of microglial CD45 and CD68 markers. This study demonstrates that, using these methods, dopamine neuronal replacement cell therapy can be beneficial for patients with advanced disease, and that changing technical approaches could have a favourable impact on efficacy and adverse events following neural transplantation.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Suède</li><li>États-Unis</li></country><region><li>Québec</li></region><settlement><li>Montréal</li></settlement></list><tree><country name="Suède"><noRegion><name sortKey="Kirik, Deniz" sort="Kirik, Deniz" uniqKey="Kirik D" first="Deniz" last="Kirik">Deniz Kirik</name></noRegion><name sortKey="Bjorklund, Anders" sort="Bjorklund, Anders" uniqKey="Bjorklund A" first="Anders" last="Björklund">Anders Björklund</name></country><country name="Canada"><noRegion><name sortKey="Mendez, Ivar" sort="Mendez, Ivar" uniqKey="Mendez I" first="Ivar" last="Mendez">Ivar Mendez</name></noRegion><name sortKey="Dagher, Alain" sort="Dagher, Alain" uniqKey="Dagher A" first="Alain" last="Dagher">Alain Dagher</name></country><country name="États-Unis"><noRegion><name sortKey="Sanchez Pernaute, Rosario" sort="Sanchez Pernaute, Rosario" uniqKey="Sanchez Pernaute R" first="Rosario" last="Sanchez-Pernaute">Rosario Sanchez-Pernaute</name></noRegion><name sortKey="Bjorklund, Lars" sort="Bjorklund, Lars" uniqKey="Bjorklund L" first="Lars" last="Björklund">Lars Björklund</name><name sortKey="Cooper, Oliver" sort="Cooper, Oliver" uniqKey="Cooper O" first="Oliver" last="Cooper">Oliver Cooper</name><name sortKey="Ferrari, Daniela" sort="Ferrari, Daniela" uniqKey="Ferrari D" first="Daniela" last="Ferrari">Daniela Ferrari</name><name sortKey="Isacson, Ole" sort="Isacson, Ole" uniqKey="Isacson O" first="Ole" last="Isacson">Ole Isacson</name><name sortKey="Vinuela, Angel" sort="Vinuela, Angel" uniqKey="Vinuela A" first="Angel" last="Vinuela">Angel Vinuela</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B43 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002B43 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Canada
|area= ParkinsonCanadaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:05-0329433
|texte= Cell type analysis of functional fetal dopamine cell suspension transplants in the striatum and substantia nigra of patients with Parkinson's disease. Commentary
}}
| This area was generated with Dilib version V0.6.29. |  |